Spectrophotometric determination of blood oxygen.
نویسندگان
چکیده
Gasometric methods for estimating blood oxygen are time-consuming. This consideration often limits the number of observations which can be made in the course of experiments requiring oxygen determinations. In many cases it is an arteriovenous difference which is particularly desired, as in the measurement of cardiac output by the Fick principle, or the estimation of oxygen consumption by human liver, kidney, or brain. The present paper describes a rapid, simple spectrophotometric method for determination of arteriovenous oxygen differences with an accuracy satisfactory for ordinary needs. The absolute oxygen content can also be estimated, subject to error caused by “inactive” hemoglobin. The method is based upon the principle that two well hemolyzed samples of blood drawn nearly simultaneously from the same individual will have substantially the same total hemoglobin content, “inactive” hemoglobin (l), turbidity, and content of other light-absorbing material; and that, consequently, the difference in optical densities of the two samples at an appropriate wave-length will be proportional to the difference in oxyhemoglobin concentration of the two samples. This hypothesis has been tested on samples of blood by comparison of differences in optical density with differences in oxygen content, determined gasometrically by the method of Van Slyke and Neil1 (2). The difference in absorption spectra of reduced and oxygenated hemoglobin has been the basis of several methods for determining per cent saturation of blood samples. Much of the basic work in this field was done by Drabkin and his colleagues, who devised a special cell with a short light path, demonstrated the applicability of the Beer-Lambert law to concentrated solutions of hemoglobin and hemoglobin derivatives (3), and measured the per cent of oxygen saturation of undiluted arterial blood samples in man and of circulating arterial blood in the dog (4). Before Drabkin’s work Hall had developed a spectroscopic method for determination of per cent saturation of hemoglobin in dilute solutions (5). This was later extended by the use of an ingenious tonometer to measurements of oxygen saturation in whole blood (6). Early work on
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عنوان ژورنال:
- The Journal of biological chemistry
دوره 180 1 شماره
صفحات -
تاریخ انتشار 1949